leptin receptor rabbit polyclonal antibody (Bioss)
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Bioss
leptin receptor rabbit polyclonal antibody
Leptin Receptor Rabbit Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leptin receptor rabbit polyclonal antibody/product/Bioss
Average 94 stars, based on 8 article reviews
Leptin Receptor Rabbit Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/leptin receptor rabbit polyclonal antibody/product/Bioss
Average 94 stars, based on 8 article reviews
leptin receptor rabbit polyclonal antibody - by Bioz Stars,
2026-03
94/100 stars
Images
1) Product Images from "Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells"
Article Title: Chemerin Stimulates the Secretory Activity of BME-UV1 Bovine Mammary Epithelial Cells
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms25084147
Figure Legend Snippet: Viability of BME-UV1 bovine mammary epithelial cells treated for 24 h with different concentrations of chemerin, leptin, or adiponectin. Graphs present cell viability measured using the MTT assay. The viability of untreated control cells (0) was designated as 100%. Results are presented as means ± standard deviation of three independent experiments.
Techniques Used: MTT Assay, Control, Standard Deviation
Figure Legend Snippet: Immunofluorescence staining of leptin receptor in BME-UV1 bovine mammary epithelial cells. Panels I and II present the localization of leptin receptor detected using primary antibodies (cat. no. bs-0961; Bioss Antibodies), followed by secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); nuclei were counterstained with 7-amino actinomycin (7-AAD, red fluorescence). Panel III presents no primary antibody control. Micrographs were taken at 600× magnification. Scale bar: 20 μm.
Techniques Used: Immunofluorescence, Staining, Fluorescence, Control
Figure Legend Snippet: Evaluation of apoptosis in BME-UV1 bovine mammary epithelial cells treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL) for 24 h. ( A ) Representative dot-plots of Annexin V/PI double staining in untreated control cells; ( B ) graph showing percentage of apoptotic cells (sum of Annexin V pos /PI neg and Annexin V pos /Pi pos cells) in control and adipokine-treated cells; ( C ) representative images of Western blot (WB) analysis of apoptotic markers cleaved caspase 3 and bax, where gapdh was used as a reference protein; ( D , E ) graphs showing the results of the densitometric analysis of WB images; the integrated optical density (IOD) of cleaved caspase 3 and bax bands was normalized to the IOD of gapdh bands. Results are presented as means ± standard deviation of three or four independent experiments.
Techniques Used: Double Staining, Control, Western Blot, Standard Deviation
Figure Legend Snippet: Concentration of αS1-casein in BME-UV1 cells ( A ) and media ( B ) collected after 24 h of culture. Cell were treated with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL) or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Untreated cells were used as a negative control, whereas cells exposed for 24 h to the lactogenic hormone prolactin (PRL, 1 µg/mL) were used as a positive control. Results are presented as means ± standard deviation of three independent experiments. Means followed by a common letter are not significantly different according to one-way ANOVA with Tukey’s multiple comparison post-test, at the 5% level of significance ( p < 0.05).
Techniques Used: Concentration Assay, Negative Control, Positive Control, Standard Deviation, Comparison
Figure Legend Snippet: Panels of confocal micrographs presenting the immunofluorescence staining of BME-UV1 cells cultured on Matrigel for 11 days in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). Cells were stained with primary antibodies against β1-integrin and secondary antibodies conjugated with Alexa Fluor 488 dye (green fluorescence); Alexa Fluor 594 phalloidin, detecting F-actin (red fluorescence), and nuclei counterstained with Hoechst 33342 (blue fluorescence). Images were taken at 600× magnification and are representative for three independent experiments. Scale bar: 20 μm.
Techniques Used: Immunofluorescence, Staining, Cell Culture, Control, Fluorescence
Figure Legend Snippet: Diameters of 3D mammospheres formed by BME-UV1 cells cultured on Matrigel for 11 days. Cells were cultured in control growth medium (Ctrl.) or medium supplemented with chemerin (Ch10 = 10 ng/mL, Ch100 = 100 ng/mL), leptin (L10 = 10 ng/mL, L100 = 100 ng/mL), or adiponectin (A10 = 10 ng/mL, A500 = 500 ng/mL). The diameters of cross sections of the spheroids were measured using ImageJ software ( https://ij.imjoy.io accessed on 2 April 2024). Results are presented as means ± standard deviation of at least 14 spheroids per experimental condition photographed using a confocal microscope equipped with a digital camera.
Techniques Used: Cell Culture, Control, Software, Standard Deviation, Microscopy
Figure Legend Snippet: List of antibodies used in the study (IF—immunofluorescence staining; WB—Western blot analysis).
Techniques Used: Staining
